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Hypothyroidism alone can lead to myocardial fibrosis and result in heart failure, but traditional hormone replacement therapy does not improve the fibrotic situation. Hydrogen sulfide (H 2 S), a new gas signaling molecule, possesses antiinflammatory, antioxidant, and anti-fibrotic capabilities. Whether H 2 S could improve hypothyroidism-induced myocardial fibrosis are not yet studied. In our study, H 2 S could decrease collagen deposition in the myocardial tissue of rats caused by hypothyroidism. Furthermore, in hypothyroidism-induced rats, we found that H 2 S could enhance cystathionine-gamma-lyase (CSE), not cystathionine β-synthase (CBS), protein expressions. Finally, we noticed that H 2 S could elevate autophagy levels and inhibit the transforming growth factor-β1 (TGF-β1) signal transduction pathway. In conclusion, our experiments not only suggest that H 2 S could alleviate hypothyroidism-induced myocardial fibrosis by activating autophagy and suppressing TGF-β1/ SMAD family member 2 (Smad 2) signal transduction pathway, but also show that it can be used as a complementary treatment to conventional hormone therapy.
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Objective To compare predicting validity of general movements (GMs) assessment for neurodevelopment outcome of high-risk premature infants in different gestational age. Methods 180 participants were divided into group A (28 to 31+6 gestational weeks), group B (32 to 33+6 gestational weeks) and group C (34 to 36+6 gestational weeks) according to gestational age, and evaluated by GMs assess-ments in the stage of writhing-movement and fidgety-movement respectively. They were evaluated with GMs assessment and Gesell devel-opmental scale, and cerebral palsy was diagnosed clinically. Results 133 infants were normal and 47 infants with developmental delay, in which 10 were with cerebral palsy. The sensitivity was 40.4%, the specificity was 90.2%, the positive predictive value was 59.4%, the nega-tive predictive value was 81.1% in the stage of writhing-movement; and it was 27.7%, 99.2%, 92.9% and 79.5% in the stage of fidg-ety-movement, respectively. There was no significantly difference in the detection rate among different age groups (P=0.610, P=0.765, P=0.290). Conclusion The absent fidgety-movement (F-) is the highest, and cramped-synchronized (CS) is the secondly to predict the motor de-velopmental outcome. There was no difference of GMs assessment to detect the neurodevelopmental outcome in premature infants of 28 to 36+6 gestational weeks.
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Objective To observe the effect of sensory integration training on motor function of cerebral palsy children.Methods 42 children with cerebral palsy were divided into two groups,with 22 cases in observation group,20 cases in control group.All children were treated with mainly Bobath technique based rehabilitation therapy,while those of observation group were added sensory integration training for 3 months.We used GMFM-88 to estimate the gross motor function of the two groups.Results After treatment,the scores of GMFM-88 significantly improved in both groups,GMFM scores of the observation group were more effective than thoes of the control group(t =2.481,P =0.018).Conclusion Sensory integration training based on tradition neural movement therapy can improve gross motor function in children with cerebral palsy,can significantly improve the curative effect of rehabilitation.
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@#Objective To observe the effect of scalp acupuncture combined with speech training on language development delay in mental retardation children. Methods 40 cases were divided into 2 groups, 20 cases in each group. The observation group was treated with scalp acupuncture combined with speech training. The control group was treated with speech training. They were assessed with the development quotient (DQ) of adaptability and language of Gesell Developmental Schedules before and 3 months after the treatment. Results DQ of adaptability and language improved after treatment in both groups (P<0.05), and improved more in the observation group than in the control group (P<0.05). Conclusion Scalp acupuncture combined with the speech training is more effective on language development delay in mental retardation children.
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@#ObjectiveTo explore the effect of oral training on speech for mental retardation child. Methods40 cases were divided into 2 groups, 20 cases in each group. The control group was treated with language cognitive training. The observation group was treated with language cognitive combined with oral training. The treatment was given for 3 months. Sign-significance Test (S-S) was used to evaluate the language development before and after treatment. ResultsThe language development quotient significantly improved after treatment (P＜0.05), and the language expression quotient of the observation group improved more than that in the control group (P＜0.05), but the language comprehension quotient and operation quotient of the both were no significantly different. ConclusionLanguage cognitive combined with oral training is more effective on language expression capability of mental retardation child.
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<p><b>AIM</b>To determine the possible roles of the t-complex testis expressed gene 5 (Tctex5) on sperm functions, the full-length sequence of mRNA was studied and compared in the testis between the normal wild-type and the sterile t-haplotype mutant mice.</p><p><b>METHODS</b>We applied rapid amplification of cDNA ends, Northern blot and reverse transcription polymerase chain reaction to analyze the full length of Tctex5 mRNAs isolated from testes of the wild-type and the t-haplotype mice. Reverse transcription polymerase chain reaction was used to semi-quantitatively compare expression of Tctex5 transcripts in the 16 tissues and 9.5 day stage embryos in the wild-type mice. E-translation was applied to estimate the amino acid sequences.</p><p><b>RESULTS</b>One long and one short transcript of Tctex5 mRNA were discovered in mouse testis of wild-type (Tctex5(long-+) and Tctex5(short-+)) and t-haplotype (Tctex5(long-t) and Tctex5(short-t)) mice, respectively. Being enhanced only in the testis, Tctex5(long-t) had 17 point mutations and one 15-bp-deletion in the exon 1 region, comparing with the Tctex5(long-+), whereas the Tctex5(short-t) was similar to the Tctex5(short-+). The short isoforms of Tctex5 mRNAs in the two models encoded exactly the same peptides, but the long isoforms did not. The estimated peptide encoded by Tctex5(long-t) had significant mutations on putative sites of phosphorylation and PP1 binding.</p><p><b>CONCLUSION</b>We established that mutations that occur in the Tctex5 long transcript of the t-haplotype mice are important for normal sperm function, whereas the short transcript of Tctex5 might have a conserved function among different tissues.</p>
Subject(s)
Animals , Male , Mice , Gene Expression , Haplotypes , Infertility, Male , Microtubule-Associated Proteins , Chemistry , Genetics , Mutation , Nuclear Proteins , Chemistry , Genetics , Protein Phosphatase 1 , Sequence Analysis, Protein , Spermatozoa , Metabolism , Testis , Metabolism , t-Complex Genome RegionABSTRACT
<p><b>OBJECTIVE</b>To explore the value of electroencephalogram (EEG) in early diagnosis of brain injury in neonates with asphyxia.</p><p><b>METHODS</b>EEG examination was performed in 49 neonates with asphyxia (mild: n=9; severe: n=40) within 6 hrs of their births. Of the 49 asphyxiated neonates, 33 had concurrent HIE, including 20 cases of mild, 9 cases of moderate and 4 cases of severe HIE.</p><p><b>RESULTS</b>Twenty-one (63.6%) out of the 33 patients with HIE showed abnormal EEG, but only one (6.3%) in the asphyxia group without HIE. All of 13 patients with moderate-severe HIE showed abnormal EEG. The degree of EEG abnormality in neonates with HIE was consistent with the clinical grading of HIE. The neonates whose EEG showed electrical silence and burst suppression and the abnormalities were kept unrecoverable for more than 2 weeks had very poor prognosis.</p><p><b>CONCLUSIONS</b>EEG can reflect brain injury caused by neonatal asphyxia and the severity of brain injury. It may be useful for early diagnosis of brain injury following asphyxia in neonates.</p>